Composite
Rep

Part:BBa_K125800:Design

Designed by: Margaret Ruzicka   Group: iGEM08_Hawaii   (2008-08-29)

Origin of Replication with Replication Proteins


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RepB' is placed under the regulation of I14032, a lac promoter and an RBS with an efficiency of 0.6. The copy number will have to be determined. RSF1010 plasmids are low copy (~10 per cell in PCC6803 and ~12 per cell in E. coli. Katashinka (2007) suggests that if RepB' is not regulated, the copy number will triple.

Source

This part is taken from the RSF1010 derived plasmid pRL1383a. A description of this plasmid can be found at the NCBI database at accession number AF403426.

References